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2 edition of Analysis of the osteoblast lineage found in the catalog.

Analysis of the osteoblast lineage

Kursad Turksen

Analysis of the osteoblast lineage

use of a cell culture model and monoclonal antibodies

by Kursad Turksen

  • 372 Want to read
  • 29 Currently reading

Published by K. Turksen in [S.l.] .
Written in English


Edition Notes

Thesis (Ph.D.)--University of Toronto, 1991.

Statementby Kursad Turksen.
ID Numbers
Open LibraryOL18235226M

Western blot analysis is used to study the activation of extracellular signal-regulated kinase (ERK1/2). Interestingly, butein promotes the lineage commitment of mBMSCs into osteoblasts, while suppressing their differentiation into adipocytes in a dose-dependent manner. A similar effect of butein is confirmed in human (h) primary BMSCs. Osteoclast Morphology and Lineage. Lesson Summary. Osteoblasts and osteoclasts are both necessary for healthy bones, but it is the osteoclasts that enable bones to change once formed.

Search the world's most comprehensive index of full-text books. My library.   acts negative at the late stages of osteoblast differentiation de Boer et al. Bone (); van der Horst et al., JBMR (); Eijken et al. J. Cell Biochem () Mimics differentiation stage-dependent effect of RUNX2 - - Preosteoblast Immature Mature OsteocyteMesenchymal stem cell osteoblast osteoblast + +.

  Osteocytes are non-proliferative, terminally differentiated cells of the osteoblast lineage, how osteoblasts transform into osteocytes is dependent on the mode of ossification (Figure 1). They reside both in the mineralized bone matrix and in newly formed osteoid, locked inside small lacuna spaces in the hard substance of bone are smaller than. Although induced pluripotent stem cells hold promise as a potential source of osteoblasts for skeletal regeneration, the induction of pluripotency followed by directed differentiation into osteoblasts is time consuming and low yield. In contrast, direct lineage reprogramming without an intervening s .


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Analysis of the osteoblast lineage by Kursad Turksen Download PDF EPUB FB2

Osteoblast lineage. All precursors of osteoblasts can proliferate; osteoblasts are transformed to osteocytes and lining cells without further proliferation. Some data suggest that lining cells might revert to osteoblast function after parathyroid hormone stimulation.

At each stage in the lineage, apoptotic cell death is probably an alternative. Interleukin-6 (IL-6) family cytokines act via gp in the osteoblast lineage to stimulate formation of osteoclasts (bone resorbing cells), activity of osteoblasts (bone forming cells) and to.

The current knowledge of the osteoblast lineage is expanding in the area of identification of the osteoprogenitor cells, along with further defining paracrine and endocrine functions of cells of the osteoblast lineage in vivo.

All of these studies require robust methods to identify and target cells of interest. by: 8. Abstract The osteoblast-lineage Analysis of the osteoblast lineage book of cells at various stages of maturation that are essential for skeletal development, growth, and maintenance.

Over the past decade, many of the signaling cascades that regulate this lineage have been. Osteoblast lineage cells consist of osteoblasts and osteocytes. Osteoblasts produce bone matrix proteins to form osteoid and may become trapped during bone formation to further differentiate to Cited by: Using this method, we obtain samples enriched for specific cell types from the mature osteoblast lineage (osteoblasts, lining cells, i.e., quiescent osteoblasts, and osteocytes).

RNA is well preserved and following extraction and amplification can be used as input to both low and high-throughput RNA analysis formats. Osteoblast, large cell responsible for the synthesis and mineralization of bone during both initial bone formation and later bone remodeling.

Osteoblasts form a closely packed sheet on the surface of the bone, from which cellular processes extend through the.

Module 9 is a co-expression network specific to cells of the osteoblast-lineage. (A). Mean MS score for each of the 21 network modules. (B) Mean module gene expression in osteoblasts.

Systems Genetic Analysis of Osteoblast-Lineage Cells Gina Calabrese1, Brian J. Bennett2, Luz Orozco3, Hyun M. Kang4, Eleazar Eskin5, Carlos Dombret6, Olivier De Backer6, Aldons J.

Lusis3,7,8, Charles R. Farber1,9,10* 1Center for Public Health Genomics, University of Virginia, Charlottesville, Virginia, United States of America, 2Department of Genetics, University of.

In summary, our results begin to clarify the composition and role of cellular networks in the osteoblast cell lineage. Generation of a WGCNA network for bone in the HMDP A genome-wide co-expression network for bone was constructed by applying WGCNA to microarray gene expression profiles from femur samples collected on 96 HMDP strains [27,28].

In summary, we have used a systems genetics approach consisting of co-expression network analysis, eQTL analysis, genome-wide association and causality modeling in a powerful mouse genetic reference population to identify a module (M9) of co-expressed genes that play an important role in the function of osteoblast-lineage cells.

Further work on the gp YF/YF mice indicated that this increase was not intrinsic to the osteoblast lineage and depended on IL-6, indicating that osteoblast formation is coupled to that of.

Deletion of G(s)alpha early in the osteoblast lineage results in a 59% decrease in the percentage of B cell precursors in the bone marrow. Analysis of peripheral blood from mutant mice revealed a 67% decrease in the number of circulating B lymphocytes by 10 days of age.

In summary, PPR expression along the osteoblast lineage is essential for exercise to improve the material properties of cortical bone. Furthermore, the influence of PPR activation on material properties is unique to exercise and does not occur during normal growth and development.

Osteoblasts are the skeletal cells responsible for synthesis, deposition and mineralization of the extracellular matrix of bone.

By mechanisms that are only beginning to be understood, stem and primitive osteoprogenitors and related mesenchymal precursors arise in the embryo and at least some appear to persist in the adult organism, where they contribute to replacement of osteoblasts.

Osteoblast lineage cells in human bone were recently shown to colonize eroded bone surfaces and to closely interact with osteoclasts. They proved to be identical to reversal cells and are believed to differentiate into bone-forming osteoblasts thereby coupling resorption and formation.

However, they also exert catabolic activity that contributes to osteoclastic bone. The osteoblast-lineage consists of cells at various stages of maturation that are essential for skeletal development, growth, and maintenance. Over the past decade, many of the signaling cascades that regulate this lineage have been elucidated; however, little is known of the networks that coordinate, modulate, and transmit these signals.

Abstract. Cells of the osteoblast lineage occupy a central position in bone metabolism. The formation of a structurally sound skeleton, with its strength and integrity conserved by constant remodeling, and the formation as well as activation of the major bone-resorbing cell, the osteoclast, are the result of direct and indirect influences of osteoblasts.

Gene Expression by Microarray Analysis. Seven-day-old cultures were harvested to obtain GFP pos and GFP neg (at the preosteoblastic stage), and day-old cultures were used to isolate GFP GFP pos and GFP neg cells at the mature osteoblast stage. The entire process of cell culture, FACS sorting, RNA extraction, probe labeling, and microarray hybridization was.

Circulating osteoblast-lineage cells have been considered rare. This study used new methods to show that osteoblastic cells are present in large numbers. F4: Stages of osteoblast lineage cell (OLC) differentiation. Mesenchymal progenitors give rise to osteoblasts and chondrocytes and are usually marked by SOX9.

If SOX9+ cells do not differentiate into chondrocytes, they progress along an osteogenic path and as they mature, they are marked progressively by the expression of RUNX2, followed by OSX.

Abstract: The concern of chondrocyte-osteoblast transition in skeleton development has continued for more than one century. Even though many morphological and in vitro data clearly indicated osteoblast differentiation as an alternative fate choice of chondrocytes besides of undergoing apoptosis, absence of the solid in vivo observation led to this chondrocyte-osteoblast.

This chapter discusses signal transduction cascades initiated by these cytokines and their effect on osteoblast differentiation. The combined action of the signal transduction pathways induced by bone‐promoting cytokines determines the commitment of MSCs toward the osteoblast lineage and the efficiency of bone formation.